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CLOVER: CLARITY, OPTOGENETICS AND VECTOR ENGINEERING RESEARCH CENTER

Viviana Gradinaru, PI
Ben Deverman, Director

The mission of the CLARITY, Optogenetics and Vector Engineering Research (CLOVER) Center is to support tissue clearing and imaging projects, optogenetic studies, and custom gene delivery vehicle development at Caltech through technology and methodology innovation, training, and infrastructure and reagent sharing.

TECHNOLOGY DEVELOPMENT

The CLOVER Center has three main research aims:

  • Tissue clearing: We are developing improved methods for imaging through thick intact tissue samples at high resolution by rendering them optically transparent and permeable to stains, antibodies and nucleic acid probes.
  • Opsin engineering: Optogenetics uses light sensitive proteins called opsins to modulate and monitor the activity of neurons and other cellular states. We are working to extend the properties of opsins through protein engineering.
  • Customized gene delivery vehicles: We are developing custom recombinant adeno-associated viruses (AAVs) for more efficient and targeted gene transfer in vivo.

INFRASTRUCTURE/RESOURCES

  • The CLOVER center is equipped with a BSL-2 virus production facility and wet labs for molecular cloning, tissue processing and macromolecular staining procedures
  • On site use of the CLOVER Center's tissue clearing facilities and technical support
  • Collaborative custom vector development

TRAINING/WORKSHOPS

  • One-on-one consultations and training for tissue clearing techniques and viral vector production to jump start research projects
  • Focused workshops for researchers with various backgrounds and experience levels

Contact

Ben Deverman
Director
230 Beckman Institute
Mail Code 139-74
E-mail:
Prof. Viviana Gradinaru
Principal Investigator
Biology and Biological Engineering
258A Alles Laboratory
Mail Code 156-29
E-mail: